| 1. | The binding affinity of remaining hemoglobin sites for oxygen is changed by the presence of co binding .
氧在血红蛋白其余位置上的键合力被一氧化碳所改变。 |
| 2. | The high binding affinity and selectivityin conjunction with the polymers ' physical robustness positions molecular imprinted polymers ( mips ) as candidates for use as preliminary screens in drug discovery
聚合物的高亲和力、高选择性及物理性质稳定特点使得分子印迹聚合物可用于药物开发中的初筛。 |
| 3. | Correspondingly , the egf binding affinity of surface egfr and the tyrosine auto - phosphorylation of immuno - precipitated egfr were also enhanced in gntv - s / h7721 cells and reduced in gntv - as / h7721 cells
Egfr的信号通路主要有两条，一是ras - raf - mek一mapk经典途径，另一条是新发现的pi一3k一pkb途径。 |
| 4. | Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen
El旧a拐成惴现2个克隆的可容牲mg7seem明显的抗原结合舌队其a分别为0 832和0 912 ，均高出附性对照（ 0 |
| 5. | Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen , the positive dones possessing apparent inhibitory effect were singled out for later use
X阳性克到驶知烘扳原kbbjg ）浊对阳性克隆进行限制陇卧赐析（ uwi和hedlll ）鉴定三用竞争elisatoljmg7重组噬菌体抗体性克隆对mg7单扶与其相应抗原结合忙的喇率，从中j ） ed出对mg7单抗与期眩抗原结合有抑余j效应的克隆用于进一涉研究。 |
| 6. | The experimental method includes selecting pure complexes of histidine - containing or cysteine - containing materials , from c - and n - terminal group of these amino acids to link to a group which have color or fluorescence or ultraviolet absorption , elucidating their binding affinity , fluorescence or uv - visible spectrum properties with zinc at physiological concentration and to elucidate their structure in the solid state via infrared spectroscopy . with the help of the concerned the data , the analysis was done to prove whether it can be applied to the zinc detection , in other words , whether it can be used as a new fluorescence probe for zinc detection
本实验首次选用在生物体内与zn ~ ( 2 + )键合能力很突出的物质? ?组氨酸和半胱氨酸，采用类似于多肽合成的方法，在其羧基或氨基分别嫁接上一个带有标记的基团，生成稳定的共价键化合物；在此化合物中模拟生理浓度条件加入锌离子，通过红外图谱、紫外图谱或荧光图谱的变化分析锌离子对标记基团是否产生影响，再结合有关数据分析其是否适合检测锌离子，即是否可能作为新的锌离子荧光探针。 |
| 7. | Reports about great enthusiasm in the synthesis of the complexes of rare earth medicine , the interaction of the rare earth complexes with dna are very few in the world . it is expected to explore the mechanisms of interaction that are used to offer an opportunity to understand which parameters affect the binding mode and binding affinity to dna . part ii
从这些总结中了解到，人们之所以选择芦丁作为小分子配体，是由于芦丁所具有的特性：它属于黄酮类化合物，具有独特的药用价值及其临床应用价值，更令人兴奋的是它还具有良好的电活性，这样就可以利用电化学方法来研究其作用机理。 |
| 8. | Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。 |
